Quantification of human immunodeficiency virus type 1 p24 antigen and antibody rivals human immunodeficiency virus type 1 RNA and CD4(+) enumeration for prognosis

Citation
Js. Read et al., Quantification of human immunodeficiency virus type 1 p24 antigen and antibody rivals human immunodeficiency virus type 1 RNA and CD4(+) enumeration for prognosis, PEDIAT INF, 19(6), 2000, pp. 544-551
Citations number
30
Categorie Soggetti
Clinical Immunolgy & Infectious Disease
Journal title
PEDIATRIC INFECTIOUS DISEASE JOURNAL
ISSN journal
08913668 → ACNP
Volume
19
Issue
6
Year of publication
2000
Pages
544 - 551
Database
ISI
SICI code
0891-3668(200006)19:6<544:QOHIVT>2.0.ZU;2-C
Abstract
Background. The sensitivity, specificity and positive predictive value of b aseline serum concentrations of HIV-1 immune complex-dissociated (ICD) p24 antigen for predicting disease progression and mortality were assessed and compared with results obtained for HIV-1 ICD p24 antigen with HIV-1 p24 ant ibody and for HIV-1 RNA with CD4(+) lymphocyte percent. Methods. Data from HIV-infected children enrolled in a North American clini cal trial (National Institute of Child Health and Human Development Intrave nous Immunoglobulin Clinical Trial) were analyzed. Disease progression was defined as growth failure, CD4(+) lymphocyte percent decline to <15% after study entry or development of an AIDS-defining opportunistic infection. Results. Baseline samples were available for ICD p24 antigen testing (media n concentration, 319 pg/ml; range, <50 to 15 640) in 240 children. The comb ination of detectable ICD p24 antigen and low p24 antibody was more sensiti ve but less specific than the combination of high HIV-1 RNA and low CD4(+) lymphocyte percent in predicting disease progression and mortality. Using r eceiver operating characteristic curves, the specificity of ICD p24 antigen with p24 antibody for classifying children's disease progression or mortal ity was as great as, or greater than, HIV-1 RNA with CD4(+) lymphocyte perc ent at points on the curve corresponding to higher sensitivity, Conclusions, The use of ICD p24 antigen with p24 antibody to identify child ren at high risk of disease progression or mortality could be a viable alte rnative to the more expensive and technically difficult HIV-1 RNA and CD4() lymphocyte assays in resource-poor settings, including developing countri es where the majority of children with HIV-1 infection reside.