Indirect organogenesis in Millingtonia hortensis Linn. f. from nodal callus

Callus was obtained from the nodal region of the explants collected from ma ture trees, when cultured on MS medium supplemented with high concentration of 6-benzyladenine (BA, 5.0 mgl(-1)) and a low concentration of indole-3-b utyric acid (IBA, 0,5 mgl(-1)). As many as 10 shoots per 1.0 cm(2) callus p iece formed on reducing the auxin lelvel in the medium to 0.2 mgl(-1). Thes e shoots were allowed to elongate up to 2-3 cm high on the same medium and then excised and transferred for further elongation to a medium containing BA (2 mgl(-1)), 1-naphthalene acetic acid (NAA, 0.5 mgl(-1)) and activated charcoal (AC, 0.3% w/v). Regenerated shoots (7-8 cm high) were transferred to MS medium containing IBA (2 mgl(-1)) and NAA (0.1 mgl(-1)) for rooting. Rooted shoots were transferred to plastic pots containing vermiculite, mois tened with 1/4(th) basal medium and maintained in a humid chamber for accli matization and hardening during two weeks.