Genetic diversity and phylogenetic relationships among accessions of hop, Humulus lupulus, as determined by amplified fragment length polymorphism fingerprinting compared with pedigree data
S. Seefelder et al., Genetic diversity and phylogenetic relationships among accessions of hop, Humulus lupulus, as determined by amplified fragment length polymorphism fingerprinting compared with pedigree data, PLANT BREED, 119(3), 2000, pp. 257-263
DNA fingerprinting using amplified fragment length polymorphisms (AFLPs) wa
s successfully employed to detect genetic relationships and variability amo
ng 90 hop cultivars and breeding lines comprising a collection of the world
's hop germplasm. Seven AFLP primer combinations produced a total of 347 fr
agments of which 151 (43.5%) were polymorphic. One-hundred and thirty infor
mative, highly reproducible DNA polymorphisms were used to estimate the gen
etic similarity (GS) which varied between 1.0 (e.g. 'Saazer' vs. 'Tettnange
r') and 0.17 ('Columbus' vs. 'Tettnanger', 'Spalter' and 'Saazer'). UPGMA (
unweighted pair-group method with arithmetic averages) clustering revealed
two main clusters, reflecting the two main sources of origin and the two ma
in breeding objectives: one cluster of mainly European origin representing
the aroma pool and a second cluster associating accessions with European ge
rmplasm infiltrated by wild American genes with less aroma quality, but a h
igher bittering potential. Each main branch was composed of four or three s
ubclusters with subgroups, respectively. Assignment of almost all genotypes
in the dendrogram was consistent with the pedigree data as far as they are
known. Consequently, AFLPs are shown to be suitable for assessing the gene
tic variability in hop germplasm and are useful for describing the genetic
relationships among cultivars and accessions, which allows phylogenetic que
stions to be addressed.