Simplified clonal multiplication of mulberry using liquid shake culture

Shoot apex and axillary bud explants of mulberry (Morus indica) were cultur ed in liquid Murashige and Skoog medium containing N-(2-chloro-4-pyridyl)-N '-phenylurea (a urea-type cytokinin) at 0.5-2 mg l(-1). The cultures were m aintained at 120 rpm up to 6 weeks. During the first 4 weeks, shoot inducti on and shoot elongation were observed, followed by adequate rooting during the latter 2 weeks in both explants. A single-step liquid medium which indu ces consecutive development of shoots and roots in a same medium is useful for effective multiplication of mulberry plantlets with reduced labor and c ost.