Valeriana jatamansi Jones is an important medicinal plant. This wild herb i
s being exploited for its roots and rhizomes which contain valepotriates, w
hich are highly effective against leprosy. The aim of this study was to est
ablish a practical method for rapid and large-scale multiplication of V. ja
tamansi by induction of shoot proliferation from shoot buds. The sterilized
explants were established on solid medium supplemented with benzyl adenine
alone or in combination with indole-acetic acid or naphthalene acetic acid
. The buds cultured on nutrient medium supplemented with BA and IAA or NAA
formed shoots, which after 3-4 weeks produced roots on the same medium. One
hundred per cent survival was obtained on hardening and field establishmen
t of well rooted shoots.