Other natural hosts of Potato virus T

Potato virus T (PVT), a member of the genus Trichovirus, was isolated from leaves of naturally Infected ulluco (Ullucus tuberosus), oca (Oxalis tubero sa), and mashua (Tropaeolum tuberosum,. These Andean tuber crops are often grown in small plots in association with potato (Solanum tuberosum) in the Peruvian highlands. PVT isolates from ulluco, oca, mashua, and potato infec ted virus-free ulluco, oca, and potato genotypes by mechanical inoculation. The Incidence of PVT in mashua, oca, and ulluco accessions from the Intern ational Potato Center (CIP) in vitro germplasm bank was less than 10%. A po lymerase chain reaction (PCR) product of approximately 330 hp was obtained from each of the four isolates using primers designed from the published PV T sequence, Restriction enzyme digestions of the PCR product did not demons trate variability.