The effects of methyleugenol (ME) on guinea-pig isolated ileum were studied
. ME reversibly relaxed basal tonus (EC50 of 52.2 +/- 18.3 mu M), an action
unaltered by 0.5 mu M tetrodotoxin or 0.5 mM hexamethonium, and relaxed th
e ileum precontracted with 60 mM KCI. ME slightly hyperpolarised the ileum
from -57.3 to -62.8 mV but had no effect in depolarised tissues. ME inhibit
ed contractions induced by ACh, histamine and KCl with IC50 values of appro
ximately 82, 124, and 65 mu M, respectively. Our results suggest that ME in
duces relaxation of guinea-pig ileum by a direct action on smooth muscle vi
a a mechanism largely independent of alterations of membrane potential (E-m
).