Bioactive compounds from Celaenodendron mexicanum

Bioactivity-directed fractionation of the CHCl3-MeOH extract of the leaves of Celaenodendron mexicanum by means of the brine shrimp lethality test and chromatographic techniques led to the isolation of three carboxylic acid t riterpenes, the new tirucalla-type triterpene, 3 alpha-hydroxytirucalla-7,2 4Z-dien-26-oic acid, 3-oxotirucalla-7,24Z-dien-26-oic acid, and epi-oleanol ic acid, and three biflavonoids amentoflavone, podocarpusflavone A, and pod ocarpusflavone B. Four non-active compounds friedelin, maytensifolin B, 3 b eta-hydroxyfriedelan-16-one, and celaenodendrolide were also obtained. epi- Oleanolic acid was the most active against brine shrimps with LC50 value of 23.3 mu M in addition, all isolates were tested for in vitro antiprotozoal and cytotoxic activities. 3-Oxotirucalla-7,24Z-dien-26-oic acid and epi-ol eanolic acid showed the highest activity against Leishmania donovani promas tigotes with IC50 values of 13.7 and 18.8 mu M, respectively Only 3-oxotiru calla-7,24Z-dien-26-oic acid showed activity against Trypanosoma brucei bru cei bloodstream forms with IC50 value of 16.8 mu M.