EFFICIENT CRE-LOX LINEARIZATION OF BACS - APPLICATIONS TO PHYSICAL MAPPING AND GENERATION OF TRANSGENIC ANIMALS

Due to the size of BAC, PAC and P1 clones, it is often difficult to co nstruct detailed restriction maps, with large number of restriction fr agments leading to ambiguity of mapping data. We report the use of Cre recombinase to linearise and asymmetrically introduce label at the un ique loxP site of large loxP-containing clones. Subsequent partial dig estion allows the direct ordering of restriction fragments. Additional ly, BAC DNA linearised using the Cre-lox system has been used successf ully to generate transgenic animals.