ORDERED DIFFERENTIAL DISPLAY - A SIMPLE METHOD FOR SYSTEMATIC COMPARISON OF GENE-EXPRESSION PROFILES

A method for display of 3'-end restriction fragments of cDNAs is propo sed, extending the idea reported recently. First, representative pools of such fragments are selectively amplified using PCR suppression eff ect. Then, simplified subsets of these fragments suitable for comparis on by PAGE are amplified by adapter-specific primers extended by two r andomly picked bases at their 3' ends. By testing all possible combina tions of extended primers the whole mRNA pool may be systematically in vestigated. The method was applied to search for molecular regional ma rkers of freshwater planarian Dugesia tigrina.