Field detection of Francisella tularensis

Citation
Bp. Berdal et al., Field detection of Francisella tularensis, SC J IN DIS, 32(3), 2000, pp. 287-291
Citations number
12
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES
ISSN journal
00365548 → ACNP
Volume
32
Issue
3
Year of publication
2000
Pages
287 - 291
Database
ISI
SICI code
0036-5548(2000)32:3<287:FDOFT>2.0.ZU;2-3
Abstract
A field investigation was undertaken following an outbreak of water-borne t ularemia in Northern Norway. Francisella tularensis bacterial cellular comp onents were analysed by rapid immunochromatography (RI)-testing, enzyme-lin ked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), Water from 1 reservoir, fed from a rapid stream, tested negative, From another re servoir, 2 of a chain of 3 wells tested negative. The third well, at the en d of the chain, contained lemming (Lemmus lemmus) carcasses and gave ample proof of F. tularensis contamination, We concluded that the origin of the o utbreak was dead, infective lemming carcasses in the water sources, For the various sampling materials, the RI-test proved itself particularly handy a nd versatile, compared with the ELISA and the PCR.