RELATIONSHIP BETWEEN THE HYDROPHOBICITY AND SPECIFIC HYDROPHOBIC SURFACE-AREA OF PESTICIDES DETERMINED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COMPARED WITH REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY
T. Cserhati et E. Forgacs, RELATIONSHIP BETWEEN THE HYDROPHOBICITY AND SPECIFIC HYDROPHOBIC SURFACE-AREA OF PESTICIDES DETERMINED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COMPARED WITH REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY, Journal of chromatography, 771(1-2), 1997, pp. 105-109
Citations number
16
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The hydrophobicity and specific hydrophobic surface area of 12 commerc
ial pesticides have been determined by reversed-phase high-performance
liquid chromatography (RP-HPLC) using octadecyl-bonded silica support
and water-methanol mixtures as eluents. The pesticides showed regular
retention behaviour, their log k' values decreased linearly with incr
easing concentration of methanol in the eluent. The hydrophobicity and
specific hydrophobic surface area of pesticides were significantly in
tercorrelated, although the chemical structures of the pesticides were
different. Principal component analysis proved that the hydrophobicit
y parameters determined by RP-HPLC and reversed-phase thin-layer chrom
atography are slightly different and herbicides and fungicides could n
ot be distinguished according to their hydrophobicity parameters. This
indicates that differences in the biological activity of these pestic
ides cannot be attributed to either their hydrophobicity or specific h
ydrophobic surface area alone.