ENANTIOSEPARATION IN CAPILLARY ELECTROPHORESIS USING 2-HYDROXYPROPYLTRIMETHYLAMMONIUM SALT OF BETA-CYCLODEXTRIN AS A CHIRAL SELECTOR

The resolving ability of the 2-hydroxypropyltrimethylammonium salt of beta-cyclodextrin (TMA-beta-CD) as a chiral selector in capillary elec trophoresis (CE) is reported in this work in continuation of our resea rch on this subject [Chem. Sec. Rev., 25 (1996) 141; J. Chromatogr. A, 732 (1996) 143, 183]. The composition of the chiral selector and the effect of its addition to the background electrolyte on the EOF in a f used-silica and a polyacrylamide-coated capillary were studied. The en antioseparations of neutral chiral analytes are reported first using a positively charged cyclodextrin (CD) derivative. The carrier ability of a positively charged CD derivative is used for the migration of neu tral analytes in a polyacrylamide-coated capillary. Several general ad vantages of this mode of electrophoretic separations are illustrated t ogether with selected possibilities for the reversal of the enantiomer migration order for neutral and anionic analytes.