C. Vaccher et al., CHIRAL RESOLUTION OF 4-SUBSTITUTED PYRROLIDIN-2-ONES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON CELLULOSE AND AMYLOSE CHIRAL STATIONARY PHASES, Journal of chromatography, 771(1-2), 1997, pp. 343-348
Citations number
21
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Analytical HPLC methods using derivatized cellulose and amylose chiral
stationary phases were developed for the separation of the enantiomer
s of 4-substituted pyrrolidin-2-ones. Separations were performed using
normal-phase methodology with a mobile phase of n-hexane-alcohol (eth
anol or 2-propanol) in various percentages, and a silica-based cellulo
se tris-3,5-dimethylphenylcarbamate (Chiralcel OD), or amylose tris-(S
)-1-phenylethylcarbamate (Chiralpak AS). The mobile phase and the chir
al stationary phase were varied to achieve the best resolution. The ef
fects of alcohol concentration, various aliphatic alcohols in the mobi
le phase were studied. The effects of substitution were analysed. Base
line separation was easily obtained in many cases.