A liquid-stable reagent for lactic acid levels - Application to the Hitachi 911 and Beckman CX7

We evaluated the use of nw lactate oxidase-based reagent for the determinat ion of serum and plasma lactic acid levels with the Hitachi 911 (Roche Diag nostics, Indianapolis, IN) and the Beckman CX7 (Beckman Instruments, Brea, CA). Evaluation studies demonstrated on-board stability of at least 3 month s and a calibration stability of more than 5 months. Within- and between-da y imprecision of this reagent was less than 2% for both applications. The r eagent is free of the deleterious effects of triglyceride up to bevels of 1 ,400 mg/dL (15.8 mmol/L), bilirubin to concentrations of 24.6 mg/dL (420 mu mol/L), and hemoglobin, from lysed erythrocytes, to levels of more than 0. 3 g/dL (3.0 g/L). When used on the Hitachi 911 for the determination of pla sma lactate concentrations, the reagent correlates with the Dade aca III (D ade International, Deerfield, a). When applied to the Beckman CX7 for the d etermination of serum lactate levels, the method correlates with the Beckma n method.