This paper considers the theory, assessment and prediction of microbial pro
tein synthesis in the rumen. The difficulties of techniques for assessing m
icrobial protein synthesis, as well as the complexity of the rumen ecosyste
m, have limited progress. Inconsistencies in the literature undermine the v
alue of incorporating advanced rumen models into rationing schemes and limi
t the exploitation of microbial protein as an important protein resource fo
r ruminants. The paper gives examples of situations in which particular fac
tors have significant effects on microbial protein synthesis, but moves on
to discuss the development of new less-invasive approaches for estimating m
icrobial protein synthesis. The latter approaches have the attraction of of
fering in-built technology transfer through the development of diagnostic t
ests, based on samples of milk or urine. Some of these techniques offer a d
escription of rumen function that is less rigorously quantitative (in terms
of microbial protein synthesis), but more usefully qualitative (in terms o
f microbial populations, substrates and interactions). (C) 2000 Elsevier Sc
ience B.V. All rights reserved.