The effect of bladder outlet obstruction on tissue oxygen tension and blood flow in the pig bladder

Objective To investigate the effect of partial bladder outlet obstruction o n detrusor blood flow and oxygen tension (PdetO(2)) in female pigs. Materials and methods Detrusor-layer oxygen tension and blood now were meas ured using oxygen-sensitive electrode and radiolabelled microsphere techniq ues in five female Large White pigs with a partial urethral obstruction and in five sham-operated controls. The effects of chronic outlet obstruction on bladder weight, and cholinergic nerve density and distribution, are also described. Results In the obstructed bladders, blood flow and oxygen tension were, res pectively, 54.9% and 74.3% of control values at low bladder volume, and 47. 5% and 42.5% at cystometric capacity. Detrusor blood flow declined by 27.8% and 37.5% in the control and obstructed bladders, respectively, as a resul t of bladder filling, whilst PdetO(2) did not decrease in the controls, but fell by 42.7% in the obstructed bladders. Bladder weight increased whilst cholinergic nerve density decreased in the obstructed animals. Conclusion In pigs with chronic bladder outlet obstruction, blood flow and oxygen tension in the detrusor layer were lower than in control animals. In addition, increasing detrusor pressure during filling caused significantly greater decreases in blood flow and oxygen tension in the obstructed than in the control bladders.