Time-resolved UV-vis spectroelectrochemical studies of the electron transfer process of cytochrome c

The present work used mainly electrochemical in situ time-resolved UV-vis s ubtractive reflectance spectroscopy to study the electron transfer process of cytochrome c in the presence of promoter (the equal molar mixture of gua nine and cytosine). Two new absorption bands 450 and 500 nm acid two bipola r bands 250 and 300 nm are observed in the spectra with different time reso lutions. In addition, the intensity of another band 420 nm changes with the time resolutions. All the results show that the electron transfer of cytoc hrome c is accompanied by significant conformational rearrangement. (C) 200 0 Elsevier Science Ltd. All rights reserved.