Electrochemical determination of lipase activity in pharmaceutical preparations

Citation
T. Fuse et al., Electrochemical determination of lipase activity in pharmaceutical preparations, ELECTR ACT, 45(18), 2000, pp. 2933-2937
Citations number
7
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
ELECTROCHIMICA ACTA
ISSN journal
00134686 → ACNP
Volume
45
Issue
18
Year of publication
2000
Pages
2933 - 2937
Database
ISI
SICI code
0013-4686(2000)45:18<2933:EDOLAI>2.0.ZU;2-Q
Abstract
Flow injection analysis with electrochemical detection was used for lipase assay in pharmaceutical preparations. The method is based on the fact that the current of voltammetric prepeak, accompanied by the reduction of 2-meth yl-1,4-naphthoquinone (vitamin K-3, VK3) due to the effects of fatty acids, is proportional to the acid concentration. A sample solution containing li pase was incubated with olive oil (substrate) at 37 degrees C with stirring for 15 min, and the released fatty acids were extracted by ether. No emuls ifying agent was needed in the incubation of the enzyme reaction mixture. A n acid test solution prepared by dissolving the extracts in ethanol contain ing 3 mM VK3 and 38 mM LiClO4 (the same composition as the carrier solution ) was injected into the flow injection system. The flow signal was linear t o the lipase activity ranging from 10 to 1500 U/l for sample solutions obta ined from the digestive preparations. By this method, analysis of the fat d igestive power of the commercially available preparations could be accompli shed with good results, thus showing this method to be practically useful. (C) 2000 Elsevier Science Ltd. All rights reserved.