Rapid up-regulation of P2Y messengers during granulocytic differentiation of HL-60 cells

HL-60 cells are human promyelocytic cells expressing two ATP receptors: the P2Y(2) and P2Y(11) subtypes. Our Northern blotting experiments have shown that P2Y(2) and P2Y(11) messengers were up-regulated in these cells, rapidl y and independently of protein synthesis, following treatment with granuloc ytic differentiating agents such as retinoic acid, dimethylsulfoxide, granu locyte-colony stimulating factor, dibutyryl cyclic AMP and ATP. AR-C67085 a nd adenosine 5'-O-(3-thiotriphosphate), two potent agonists of the recombin ant P2Y(11) receptor, increased intracellular cAMP concentration in HL-60 c ells more potently than ATP itself. These observations support the conclusi on that the effect of ATP on HL-60 cell differentiation is mediated by the P2Y(11) receptor. (C) 2000 Federation of European Biochemical Societies. Pu blished by Elsevier Science B.V. All rights reserved.