Methods for the detection, enumeration and identification of Alicyclobacillus acidoterrestris

Detection and enumeration of Alicyclobacillus acidoterrestris in products a nd on equipment surfaces are described, including sample heat treatment and membrane filtration to improve sensitivity. Rapid identification of A. aci doterrestris is discussed, including a pragmatic approach based on odour, b iochemical test strips, ribotyping and cell wall analysis. Practical applic ation of both cultural and microscopic methods to an investigation of a com mercial spoilage problem is described. Growth of A. acidoterrestris (vegeta tive cells or spores) in orange juice and apple juice was prevented by benz oic acid (150 mg/L), sorbate (300 mg/L) or (sic).