Elevated atmospheric CO2 alters microbial population structure in a pasture ecosystem

An increase in concentration of atmospheric CO2 is one major factor influen cing global climate change. Among the consequences of such an increase is t he stimulation of plant growth and productivity. Below-ground microbial pro cesses are also likely to be affected indirectly by rising atmospheric CO2 levels, through increased root growth and rhizodeposition rates. Because ch anges in microbial community composition might have an impact on symbiotic interactions with plants, the response of root nodule symbionts to elevated atmospheric CO2 was investigated. In this study we determined the genetic structure of 120 Rhizobium leguminosarum by, trifolii isolates from white c lover plants exposed to ambient (350 mu mol mol(-1)) or elevated (600 mu mo l-mol(-1)) atmospheric CO2 concentrations in the Swiss FACE (Free-Air-Carbo n-Dioxide-Enrichment) facility. Polymerase Chain Reaction (PCR) fingerprint ing of genomic DNA showed that the isolates from plants grown under elevate d CO2 were genetically different from those isolates obtained from plants g rown under ambient conditions. Moreover, there was a 17% increase in nodule occupancy under conditions of elevated atmospheric CO2 when strains of R. leguminosarum by. trifolii isolated from plots exposed to CO2 enrichment we re evaluated for their ability to compete for nodulation with those strains isolated from ambient conditions. These results indicate that a shift in t he community composition of R. leguminosarum by. trifolii occurred as a res ult of an increased atmospheric CO2 concentration, and that elevated atmosp heric CO2 affects the competitive ability of root nodule symbionts, most li kely leading to a selection of these particular strains to nodulate white c lover.