Microwave tomographic experiments have been performed on a three-dimensiona
l (3-D) phantom and excised canine heart using a 3-D system operating at Fr
equency of 2.4 GHz, A modified gradient reconstruction approach has been em
ployed for the 3-D image reconstruction. To compare two-dimensional (2-D) a
nd 3-D approaches, we also performed 2-D image reconstruction using an appr
oach based on the Newton method. Experimental data acquired on experimental
phantoms were analyzed using both 2-D and 3-D reconstruction approaches. H
igh-quality images were reconstructed using the 3-D approach. The reconstru
ction procedure failed when the 2-D approach was applied to reconstruct ima
ges of the 3-D object. An image of the dielectrical properties of the excis
ed canine heart was obtained using a 3-D reconstruction approach. Images su
ccessfully revealed a complex internal structure of the heart, including bo
th right-hand side and left-hand side ventricles.