Associations between HLA-DQB1 high-risk alleles and type I diabetes do notdepend on cytomegalovirus antibody statas at onset: A case-parent study conducted in Chile

The purpose of the present study is to ascertain whether the associations b etween HLA-DQBI*0201 and DeB1*0302 alleles and childhood diabetes depend on the presence of antibodies to human cytomegalovirus (CMV). A study of inci dent type I diabetes cases and parents was conducted in Santiago, Chile. HL A-DOB1 polymorphisms were determined in 85 case-parent trios (255 subjects) , while the detection of CMV was carried out only in the incident cases. As expected HLA-DQB1 polymorphisms are strongly associated with type I diabet es, with crude odds ratios of 3.7 (95% confidence interval (CI) 1.8-7.7) fo r the DeB1*0201 allele and 10.3 (95% CI 5.0-21.4) for the DQB1*0302 allele. In the subset of families with CMV+ cases, the odds ratios were estimated as 3.7 (95% CI 1.6-8.6) for the DeB1*0201 allele and 11.1 (95% CI 4.8-25.8) for the DQB1*0302 allele. In families with patients who tested negative fo r CMV antibodies, the odds ratios were calculated as 3.5 (95% CI 0.7-16.8) for the DeB1*0201 allele, and 8.0 (95% CI 1.8-34.7) for the DeB1*0302 allel e. There was no evidence of statistical interaction between CMV antibodies and the DeBI*0201 allele (P value = 0.9) or the DQB1*0302 allele (P value = 0.7). In conclusion, alleles DgB1*0302 and DeBI*0201 do not display distin ct associations with type I diabetes depending on the presence of antibodie s for CMV.