MUCUS GLYCOCONJUGATE SECRETION IN COOL AND HYPERTONIC SOLUTIONS

For sensitive individuals, exercise-induced asthma is triggered by col d and dry air and is often accompanied by a productive cough. In this study, we determined whether cold solutions and/or solutions of increa sed tonicity directly caused an increase in glycoconjugate (GC) secret ion. To test this, we used isolated swine tracheal submucosal gland ce lls (TSGCs) and measured the rate of GC secretion at 37 and 32 degrees C in isotonic solutions and in solutions made hypertonic by 30 mosM. TSGCs were isolated under conditions that minimized the rate of GC sec retion and were perfused with medium 199 equilibrated with 5% CO2 to a pH of 7.4. A lectin-based assay was used to specifically detect GC pr esent in each 2-min fraction of the perfusate. Basal secretion was 3.1 -fold greater at 32 degrees C (n = 3) than at 37 degrees C (n = 4; P < 0.05). At 37 degrees C, increasing perfusate osmolarity by 30 mosM in creased the average rate of secretion by 41 +/- 11% (n = 4; P < 0.05); return to isotonic perfusate caused a 4.5 +/- 1.8-fold transient incr ease in secretion(n = 4; P < 0.05) that was complete within 10 min. At 32 degrees C, changing tonicity of the perfusate had no significant e ffect but returning to isotonic perfusate caused a 2.3 +/- 0.7-fold tr ansient increase in secretion (n = 3; P < 0.05). Thus key stimuli that trigger obstruction of airflow (cold and increased osmolarity) can al so directly stimulate GC secretion in the airway. Such increased secre tions may contribute to the productive cough observed in some individu als in response to cold air.