MK800-62F1, a new inhibitor of apoptotic cell death, from Streptomyces diastatochromogenes MK800-62F1 - I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activity

A new compound, MK800-62F1, was isolated from a cultured broth of Streptomy ces diastatochromogenes MK800-62F1, It inhibited H2O2-induced apoptosis in human small cell lung carcinoma Ms-1 cells as well as in human T-cell leuke mia Jurkat cells. In addition, MK800-62F1 also inhibited camptothecin-induc ed apoptosis in Jurkat cells, which was mediated by intracellular H2O2 gene ration. MK800-62F1 did not exhibit antioxidative activity in vitro, suggest ing that inhibition of apoptosis by MK800-62F1 was not due to the scavengin g of H2O2, rather it was due to the modulation of the downstream event of H 2O2 generation.