A novel, rapid, and inexpensive method for the demonstration of protease inhibitory activity of legume seeds

Authors
Mulimani, VH Sudheendra, K Thippeswamy, S
Citation
Vh. Mulimani et al., A novel, rapid, and inexpensive method for the demonstration of protease inhibitory activity of legume seeds, J BIOL EDUC, 34(3), 2000, pp. 152-154
Citations number
11
Categorie Soggetti
Biology
Journal title
JOURNAL OF BIOLOGICAL EDUCATION
ISSN journal
00219266 → ACNP
Volume
34
Issue
3
Year of publication
2000
Pages
152 - 154
Database
ISI
SICI code
0021-9266(200022)34:3<152:ANRAIM>2.0.ZU;2-2
Abstract
An assay has been developed for protease activity which uses the gelatin on the surface of an unprocessed X-ray film as the proteolytic substrate. A p ositive reaction is indicated by a clear zone on the film. The method is in expensive, rapid, simple, and useful when large numbers are to be screened for their protease inhibitory activity.