Vh. Mulimani et al., A novel, rapid, and inexpensive method for the demonstration of protease inhibitory activity of legume seeds, J BIOL EDUC, 34(3), 2000, pp. 152-154
An assay has been developed for protease activity which uses the gelatin on
the surface of an unprocessed X-ray film as the proteolytic substrate. A p
ositive reaction is indicated by a clear zone on the film. The method is in
expensive, rapid, simple, and useful when large numbers are to be screened
for their protease inhibitory activity.