Characterization of commercial soybean products by conventional and perfusion reversed-phase high-performance liquid chromatography and multivariate analysis

Conventional and perfusion reversed-phase high-performance liquid chromatog raphy are used to characterize commercial soybean products for human consum ption. For this purpose, previously optimized methods of conventional and p erfusion chromatography applied to the separation of soybean proteins are e mployed. Sixty different samples corresponding to 26 different trademarks o f soybean products [soybean protein isolate, soybean flour, textured soybea n, soybean milks (liquid and powdered), and soybean infant formulas] are an alyzed. Characterization of soybean products is carried out on the basis of their protein profiles obtained by both chromatographic methods. Data obta ined are processed using multivariate methods such as principal components and discriminant analysis. Perfusion chromatography enables a further and f aster characterization of commercial soybean products than conventional chr omatography, of great value in the quality control of this kind of product. (C) 2000 Elsevier Science B.V. All rights reserved.