Development of phosphine reagents for the high-performance liquid chromatographic-fluorometric determination of lipid hydroperoxides

Phosphine reagents were designed and synthesized as a new type of fluoresce nt reagents for the determination of lipid hydroperoxides in foodstuff and biological materials. All phosphine reagents prepared had no fluorescence b ut their oxides, which were produced by the reaction of the phosphines with hydroperoxides, had strong fluorescence. Among the phosphine reagents prep ared, diphenyl-1-pyrenylphosphine had the most suitable properties as a flu orescent reagent and was successfully applied to the determination of hydro peroxides by batch, how injection and HPLC post-column methods. (C) 2000 El sevier Science B.V. All rights reserved.