beta-cyclodextrin polymer as the immobilization matrix for peroxidase and mediator in the fabrication of a sensor for hydrogen peroxide

A novel immobilization approach based on the supramolecular function betwee n beta-cyclodextrin polymer (beta-CDP) and cationic dyes, and the condensat ion polymerization among beta-CDP, glutaric dialdehyde and horseradish pero xidase (HRP) in the fabrication of a hydrogen peroxide sensor is described. IR and UV-vis spectroscopy were employed to characterize the structure of the composite membrane modified on the surface of the electrode. AFM was us ed to visualize the morphology of the composite membrane. The characteristi cs of supramolecular inclusion compounds between beta-CDP and cationic dyes were studied. The fabricated H2O2 sensor responded rapidly to H2O2 in the linear range from 1.0 to 1.1 mmol 1(-1) with a detection limit of 0.5 mol 1 (-1). (C) 2000 Elsevier Science S.A. All rights reserved.