Expression of Bcl-X-L restores cell survival, but not proliferation and effector differentiation, in CD28-deficient T lymphocytes

Citation
Am. Dahl et al., Expression of Bcl-X-L restores cell survival, but not proliferation and effector differentiation, in CD28-deficient T lymphocytes, J EXP MED, 191(12), 2000, pp. 2031-2037
Citations number
22
Categorie Soggetti
Medical Research General Topics
Journal title
JOURNAL OF EXPERIMENTAL MEDICINE
ISSN journal
00221007 → ACNP
Volume
191
Issue
12
Year of publication
2000
Pages
2031 - 2037
Database
ISI
SICI code
0022-1007(20000619)191:12<2031:EOBRCS>2.0.ZU;2-I
Abstract
Lymphocytes deficient in the T cell costimulatory molecule CD28 exhibit def ects in cell survival, clonal expansion, and differentiation into effector cells. It is known that CD28-mediated signaling results in the upregulation of the Bcl family member Bcl-X-L. To investigate the role that Bcl-X-L pla ys in the various functions of CD28, we expressed Bcl-X-L in CD28-deficient primary T lymphocytes using retrovirus-mediated gene transfer. T cells wer e activated in vitro and infected with Bcl-X-L or control retroviruses; thi s method allows gene expression in activated, cycling cells. Expression of Bcl-X-L in naive T cells was achieved by reconstitution of the immune syste m of lethally irradiated recipient mice with retrovirus-infected purified b one marrow stem cells from CD28(-/-) or wild-type donor mice. Our studies d emonstrate that Bcl-X-L prolongs the survival of CD28(-/-) T cells but does not restore normal proliferation or effector cell development. These resul ts indicate that the various functions of CD28 can be dissociated, and prov ide an experimental approach for testing the roles of downstream signals in the functions of cellular receptors such as CD28.