RNA transcripts were prepared from plasmids encoding an infectious cDNA of
foot-and-mouth disease virus (FMDV) or derivatives in which the leader (Lab
and Lb) and capsid protein coding sequences were deleted or replaced by se
quences encoding chloramphenicol acetyltransferase (CAT). The transcripts w
ere electroporated into BHK cells and the expression of CAT and the FMDV 3C
protease was monitored. Detection of CAT and 3C was dependent on the abili
ty of the transcript to replicate. All of the Lb coding sequence and 94% of
P1 (the capsid protein precursor) coding sequence could be deleted without
any apparent effect on the ability of the RNA to replicate. Thus, no cis-a
cting replication element is present within this region of the FMDV genome.
Trans-encapsidation of these FMDV replicons was very inefficient, which ma
y explain the lack of production of defective-interfering particles in FMDV
-infected cells.