The complete nucleotide sequence of fowl adenovirus type 8

The fowl adenovirus type 8 (FAdV-8) genome was sequenced and found to be 45 063 nucleotides in length, the longest adenovirus (AdV) genome for which th e complete nucleotide sequence has been determined so far. No regions homol ogous to early regions 1, 3 and 4 (E1, E3 and E4) of mastadenoviruses were recognized. Gene homologues for early region 2 (E2) proteins, intermediate protein IVa2 and late proteins were found by their similarities to protein sequences from other AdVs, However, sequences homologous to intermediate pr otein IX and late protein V could not be identified. Sequences for virus-as sociated RNA could also not be recognized. Two regions of repeated sequence s were found on the FAdV-8 genome. The shorter repeat region contained five identical and contiguous direct repeats that were each 33 bp long, while t he longer repeat region was made of 13 identical and contiguous, 135 bp lon g repeated subunits.