A O-17 NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum

Authors
Casny, M Rehder, D Schmidt, H Vilter, H Conte, V
Citation
M. Casny et al., A O-17 NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum, J INORG BIO, 80(1-2), 2000, pp. 157-160
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics","Inorganic & Nuclear Chemistry
Journal title
JOURNAL OF INORGANIC BIOCHEMISTRY
ISSN journal
01620134 → ACNP
Volume
80
Issue
1-2
Year of publication
2000
Pages
157 - 160
Database
ISI
SICI code
0162-0134(20000530)80:1-2<157:AONSOP>2.0.ZU;2-E
Abstract
The O-17 NMR of bromoperoxidase in Tris buffer at pH 8 treated with O-17-en riched H2O2 reveals direct binding of peroxide to active site vanadium both in the symmetric, and asymmetric modes, the latter possibly due to hydrope roxide. In addition, non-active site HVO2(O-2)(2)(2-) is detected. The resu lts are counter-checked with NMR data on peroxovanadium model compounds. (C ) 2000 Elsevier Science Inc. All rights reserved.