Cowpea mosaic virus infection induces a massive proliferation of endoplasmic reticulum but not Golgi membranes and is dependent on de novo membrane synthesis

Citation
Je. Carette et al., Cowpea mosaic virus infection induces a massive proliferation of endoplasmic reticulum but not Golgi membranes and is dependent on de novo membrane synthesis, J VIROLOGY, 74(14), 2000, pp. 6556-6563
Citations number
60
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
74
Issue
14
Year of publication
2000
Pages
6556 - 6563
Database
ISI
SICI code
0022-538X(200007)74:14<6556:CMVIIA>2.0.ZU;2-Y
Abstract
Replication of cowpea mosaic virus (CPMV) is associated with small membrano us vesicles that are induced upon infection. The effect of CPMV replication on the morphology and distribution of the endomembrane system in living pl ant cells was studied by expressing green fluorescent protein (GFP) targete d to the endo plasmic reticulum (ER) and the Golgi membranes. CPMV infectio n was found to induce an extensive proliferation of the ER, whereas the dis tribution and morphology of the Golgi stacks remained unaffected. Immunoloc alization experiments using fluorescence confocal microscopy showed that th e proliferated ER membranes were closely associated with the electron-dense structures that contain the replicative proteins encoded by RNA1. Replicat ion of CPMV was strongly inhibited by cerulenin, an inhibitor of de novo li pid synthesis, at concentrations where the replication of the two unrelated viruses alfalfa mosaic virus and tobacco mosaic virus was largely unaffect ed. These results suggest that proliferating ER membranes produce the membr anous vesicles formed during CPMV infection and that this process requires continuous lipid biosynthesis.