A comparison of the Bioscreen method and microscopy for the determination of lag times of individual cells of Listeria monocytogenes

Citation
Y. Wu et al., A comparison of the Bioscreen method and microscopy for the determination of lag times of individual cells of Listeria monocytogenes, LETT APPL M, 30(6), 2000, pp. 468-472
Citations number
18
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
LETTERS IN APPLIED MICROBIOLOGY
ISSN journal
02668254 → ACNP
Volume
30
Issue
6
Year of publication
2000
Pages
468 - 472
Database
ISI
SICI code
0266-8254(200006)30:6<468:ACOTBM>2.0.ZU;2-O
Abstract
Lag phase durations (t(Lag)) Of individual Listeria monocytogenes cells wer e analysed using the NightOwl Molecular Imaging System, and results were co mpared with mean individual cell lag times (t(L)) Obtained from the detecti on time (t(d)) method using Bioscreen. With Bioscreen, an average t(L) of 6 . 39 +/- 0 . 89 h was obtained from five separate experiments. With the Ni ghtOwl method, an average t(Lag) of 2 . 73 +/- 0 . 06 h was obtained from t hree experiments consisting of eight total replicates. Lag values from the NightOwl and Bioscreen are related by the equation: t(Lag) = t(L) + DT wher e DT is the doubling time. The equivalent t(Lag) mean value for the Bioscre en method was 7 . 11 +/- 0 . 84 h. Individual lag times measured by both me thods were normally distributed (r(2) for Bioscreen and NightOwl ranged fro m 0 . 951 to 0 . 999 and from 0 . 884 to 0 . 982, respectively). The result s suggest that the NightOwl method can provide accurate estimates of indivi dual cell lag times, which will facilitate the development of combined disc rete continuous models for bacterial growth.