Postglacial recolonization routes for Picea abies K. in Italy as suggestedby the analysis of sequence-characterized amplified region (SCAR) markers

The routes through which Norway spruce recolonized the Alps after the last ice age were investigated at the genetic level. Seven populations along the Alpine range plus one Apennine population were characterized for seven seq uence-characterized amplified region (SCAR) loci, detecting an overall F-ST = 0.118. This rather high value for forest species reflects an uneven dist ribution of genetic variability, and was analysed through different statist ical methods. Alternative hypotheses were tested under the isolation-by-dis tance model and using the analysis of molecular variance (amova) frame. We conclude that the hypothesis of the existence of a glacial refugium in the Apennines should be rejected, while a putative relict population is identif ied in the Maritime Alps. The Alpine range of Norway spruce appears to be s plit in two parts across a north-south line. The results are discussed in c omparison with data based on morphological markers, isozymes, chloroplast m icrosatellites and mitochondrial markers.