Detection of tau phosphorylated at threonine 231 in cerebrospinal fluid ofAlzheimer's disease patients

Authors
Kohnken, R Buerger, K Zinkowski, R Miller, C Kerkman, D DeBernardis, J Shen, JF Moller, HJ Davies, P Hampel, H
Citation
R. Kohnken et al., Detection of tau phosphorylated at threonine 231 in cerebrospinal fluid ofAlzheimer's disease patients, NEUROSCI L, 287(3), 2000, pp. 187-190
Citations number
19
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE LETTERS
ISSN journal
03043940 → ACNP
Volume
287
Issue
3
Year of publication
2000
Pages
187 - 190
Database
ISI
SICI code
0304-3940(20000630)287:3<187:DOTPAT>2.0.ZU;2-L
Abstract
A new sandwich ELISA is described which shows specificity for tau phosphory lated at threonine 231 and preferentially reacts with Alzheimer's disease ( AD) brain extracts relative to other dementias. This assay was used to anal yze 58 antemortem cerebrospinal fluid samples. Twenty-three of 27 AD sample s (85% sensitivity) yielded signals greater than the cutoff, while only one of 31 non-AD samples (97% specificity) were greater. This indicates that d etection of phosphotau in cerebrospinal fluid with this sandwich ELISA coul d prove useful in the diagnosis of AD. (C) 2000 Elsevier Science Ireland Lt d. All rights reserved.