Disposable enzyme sensors for food analysis and clinical chemistry

Enzyme sensors using various oxidases and oxidase based coupled enzyme sequ ences for the detection of glucose, sucrose, lactose,lactate, creatinine, c reatine, amino acids and biogenic amines are presented. These sensors are b ased on flexible, low cost and disposable transducers either of the laminat ed type or the screen printed type. The enzymes are immobilized onto a Pt-w orking electrode by entrapment in a hydrophilic hydrogel. Most of the basic enzyme sensors show sensitivities of 1 nA/mu M analyte. In real samples ef fects of electrochemically interfering substances on the sensor signals hav e been minimized by applying an anti-interference layer. The linear range o f the sensors has been shifted to higher concentrations by using additional diffusion limiting membranes.