Thick-film glucose biosensor in a three-electrode configuration

In this work, a glucose biosensor based on a three-electrode (counter, refe rence and working) planar configuration was fabricated using thick film tec hnology. Glucose oxidase was immobilized in the bulk of a graphite-epoxy co mposite paste in order to screen-print the working electrode. On the other hand, the intergration on the same substrate of a pseudo-reference electrod e was obtained by screen printing a commercial silver-epoxy paste, and subs equent electrochemical chlorinization. Finally, the counter electrode was i ntegrated using the same technique by depositing a graphite-epoxy composite paste, taking into account the counter/working electrode area ratio. Respo nse characteristics and reproducibility of glucose biosensor strips based o n this transducer are very promising.