MR imaging of nitrosyl-iron complexes: Experimental study in rats

PURPOSE: To assess the influence of several nitrosyl-iron complexes on prot on nuclear spin relaxation rates to establish a magnetic resonance (MR) ima ging technique for nitric oxide. MATERIALS AND METHODS: The influence of aqueous phantom solutions of nitros yl-iron complexes on proton relaxation rates was analyzed for signal enhanc ement at conventional 1.5-T MR imaging. To induce formation of nitrosyl-iro n complexes in a biologic tissue, isolated rat liver was perfused with a sa line solution of the NO donor sodium nitroprusside (SNP), and the MR signal intensity was examined thereafter RESULTS: All investigated nitrosyl-iron complexes shortened the longitudina l, or T1, and transverse, or T2, relaxation times in a concentration-depend ent fashion. Relaxivities were highest with a dinitrosyl-iron complex bound to albumin and with a water-soluble mononitrosyl-iron dithiocarbamate comp lex. The contrast properties of 240 mu mol/L of a paramagnetic nitrosyl-iro n complex were sufficient to substantially enhance the signal intensity of SNP-perfused rat livers at hydrogen 1 MR Imaging. CONCLUSION: Nitrosyl-iron complexes exhibit a contrast effect at MR imaging that can be exploited for NO imaging in living animals and patients with c onventional H-1 MR imaging techniques.