Monitoring complex bacterial communities using culture-independent molecular techniques: application to soil environment

Citation
L. Ranjard et al., Monitoring complex bacterial communities using culture-independent molecular techniques: application to soil environment, RES MICROB, 151(3), 2000, pp. 167-177
Citations number
63
Categorie Soggetti
Microbiology
Journal title
RESEARCH IN MICROBIOLOGY
ISSN journal
09232508 → ACNP
Volume
151
Issue
3
Year of publication
2000
Pages
167 - 177
Database
ISI
SICI code
0923-2508(200004)151:3<167:MCBCUC>2.0.ZU;2-3
Abstract
Over the last decade, important advances in molecular biology led to the de velopment of culture-independent approaches to describing bacterial communi ties. These new strategies, based on the analysis of DNA directly extracted from environmental samples, circumvent the steps of isolation and culturin g of bacteria, which are known for their selectivity leading to a non-repre sentative view of the extent of bacterial diversity. This review provides a n overview of the potentials and limitations of some molecular approaches c urrently used in microbial ecology. Examples of applications to the study o f indigenous soil microbial community illustrate the feasibility and the po wer of such approaches. (C) 2000 Editions scientifiques et medicales Elsevi er SAS.