Sa. Teter et Dj. Klionsky, Transport of proteins to the yeast vacuole: autophagy, cytoplasm-to-vacuole targeting, and role of the vacuole in degradation, SEM CELL D, 11(3), 2000, pp. 173-179
The vacuole/lysosome performs a central role in degradation. Proteins and o
rganelles are transported to the vacuole by selective and non-selective pat
hways. Transport to the vacuole by autophagy is the primary mode for degrad
ation of cytoplasmic constituents under starvation conditions. Autophagy ov
erlaps mechanistically and genetically with a biosynthetic pathway termed C
vt (Cytoplasm-to-vacuole targeting) that operates under vegetative conditio
ns to transport the resident vacuolar hydrolase aminopeptidase I (API). API
import has been dissected to reveal the action of a novel mechanism that t
ransports cargo within double-membrane vesicles. Recent work has uncovered
molecular components involved in autophagy and the Cvt pathway.