Absence of shedding of two B-abortus strains in goats after vaccination with live vaccines

The objective of this study was to determine if B. abortus rough mutant str ains RB51 and rfbK are eliminated in goat milk, Thirty milk goats were divi ded into two groups. Group I was inoculated with 4 x 10(10) cfu/ml of B.abo rtus RB51 strain and Group II with 1 x 10(9) cfu/ml of B. abortus rfbK stra in by subcutaneous route in the right axilary region. Milk samples were tak en aseptically on a daily basis for the first 30 days and weekly for the se cond and third months. The samples were inoculated on Farrell's media and i ncubated under 5-10% CO2 at 37 degrees C for 10 days. The suspicious coloni es were recultured in Farrell's media. There were no isolations of bacteria with characteristics of Brucella from any of the milk samples collected du ring 90 days of the study. It was concluded that neither of the strains use d at these doses were eliminated by milk in goats inoculated during lactati on. (C) 2000 Published by Elsevier Science Ltd. All rights reserved.