Both point mutation and RNA recombination contribute to the sequence diversity of citrus viroid III

Field-grown citrus trees often harbor complex mixtures of 4-5 different vir oid species, and the presence of citrus viroid III (CVd-III) has been shown to reduce the rate of tree growth without inducing disease. To more fully define the structure of its quasi-species, we have examined nine citrus vir oid complexes for the presence of previously undescribed sequence variants of CVd-III. Analysis of 86 full-length cDNAs generated from these nine viro id complexes by RT-PCR revealed the presence of 20 new CVd-III variants. Ch ain lengths ranged from 293-297 nucleotides, and sequence changes were conf ined largely to the lower portions of the central conserved region and vari able domain. The previously described variants CVd-IIIa (297 nt) and CVd-II Ib (294 nt) were clearly predominant, but phylogenetic analysis indicated t hat certain isolates may contain representatives of two additional fitness peaks. At least one group of CVd-III variants appears to have arisen as a r esult of RNA recombination. Populations recovered from diseased/declining t rees were the most diverse, but even dwarfing isolates originating from old line Shamouti trees showed considerable variability.