Nucleotide sequence of the structural protein-encoding region of foot-and-mouth disease virus A22-India

Nucleotide sequence of the structural protein-encoding region of foot-and-m outh disease virus (FMDV) A22-India 17/77 was determined using non-radioiso topic technique. Comparison of nucleotide and deduced amino acid sequence w ith A22-Iraq 24/64 revealed 175 synonymous (silent) and 42 non-synonymous n ucleotide changes resulting in 34 amino acid substitutions along the capsid proteins (VP1-VP4). Out of the 4 structural proteins VP4 is highly conserv ed. The highly variable and immunodominant protein VP1 showed 47% of the to tal amino acid substitutions. VP2 and VP3 contain 38.2% and 14.7% of the am ino acid substitutions, respectively. The VP1-based phylogenetic analysis o f 18 different type A viruses including A22-India 17/77 divided them in to two broad genetic groups (Asian and European/South American), and each grou p is further subdivided in to two separate genotypes. A22-India 17/77, A22- Iraq 24/64 and A22-Azerbaijan/65 formed one genotype and the 4 Chinese stra ins formed a separate genotype in the Asian group of viruses. In the Europe an/ South American group, A-Argentina/87 represents one genotype and the re maining 10 strains formed the second genotype in this group.