B. Siminszky et al., A cytochrome P450 monooxygenase cDNA (CYP71A10) confers resistance to linuron in transgenic Nicotiana tabacum, WEED SCI, 48(3), 2000, pp. 291-295
The isolation of a Glycine max cytochrome P450 monooxygenase (P450) cDNA de
signated CYP71A10 that. conferred linuron resistance to laboratory-grown, t
ransgenic Nicotiana tabacum seedlings was previously reported. A nonsegrega
ting transgenic N. tabacum line has been established that possesses two ind
ependent copies of the G. max CYP71A10 transgene. Five-week-old progeny pla
nts of this selected line were grown in a controlled environmental chamber
and treated with linuron using either pretransplant incorporated (PTI) or p
ostemergence (POST) applications. CYP71A10-transformed N. tabacum was more
tolerant to linuron than the wild type for both application methods. The tr
ansgenic N. tabacum line tolerated an approximately 16-fold and 12-fold hig
her rate of linuron than wild-type N, tabacum when the herbicide was applie
d PTI or POST, respectively. These results provide evidence char, plant-der
ived P450 genes can be employed effectively to confer herbicide resistance
to transgenic plants.