Effect of various carbon and nitrogen sources on cellulose synthesis by Acetobacter xylinum

The effect of various carbon and nitrogen sources on cellulose membrane pro duction by Acetobacter xylinum was evaluated. Among the carbon sources, suc rose, glucose and mannitol were found to be suitable for optimum levels of cellulose production. The strain was able to utilize a wide range of protei n and nitrogen sources such as peptone, soybean meal, glycine, casein hydro lysate, and glutamic acid for cellulose synthesis. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of pellicle proteins (PP) revealed electrophoretic bands of molecular masses in the range of 11 6-20 kDa. Furthermore, the strain can be useful for the removal of various nitrogenous and carbon substrates present in waste waters.