A mutant subtilisin E with enhanced thermostability

Authors
Yang, YH Jiang, L Yang, SL Zhu, LQ Wu, YJ Li, ZJ
Citation
Yh. Yang et al., A mutant subtilisin E with enhanced thermostability, WORLD J MIC, 16(3), 2000, pp. 249-251
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN journal
09593993 → ACNP
Volume
16
Issue
3
Year of publication
2000
Pages
249 - 251
Database
ISI
SICI code
0959-3993(200004)16:3<249:AMSEWE>2.0.ZU;2-M
Abstract
A mutant subtilisin E with remarkably thermostability is reported. It is mo re active against the typical substrate s-AAPF-pna than the wild-type subti lisin E. The time required for getting 50% residual activity of Ser236Cys s ubtilisin E at 60 degrees C in aqueous solution was approximately 80 min wh ich is 4 times longer than that of wild-type subtilisin E. Similar to the w ild-type subtilisin E, the amidase activity of Ser236Cys subtilisin E is dr amatically reduced in the presence of dimethylformamide (DMF).