The participation of ribosome-UDP-GalNAc complex in the initiation of protein glycosylation in vitro

Authors
Paszkiewicz-Gadek, A Porowska, H Gindzienski, A
Citation
A. Paszkiewicz-gadek et al., The participation of ribosome-UDP-GalNAc complex in the initiation of protein glycosylation in vitro, ACT BIOCH P, 47(2), 2000, pp. 421-426
Citations number
25
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ACTA BIOCHIMICA POLONICA
ISSN journal
0001527X → ACNP
Volume
47
Issue
2
Year of publication
2000
Pages
421 - 426
Database
ISI
SICI code
0001-527X(2000)47:2<421:TPORCI>2.0.ZU;2-F
Abstract
The gastric epithelial cells ribosome-UDP-GaPNAc complex is a donor of UDP- GalNAc as the substrate for N-acetylgalactosaminyltransferase, which cataly se the transfer of GalNAc residue to the polypeptide, existing on polysomes . It was observed that the deglycosylated porcine mucin and synthetic pepti de (PTSSPIST) can be also glycosylated with participation of N-acetylgalact osaminyltransferase and ribosome-UDP-GalNAc complex. The probability of the ribosome-UDP-GalNAc complex as an intermediate in the O-glycosylation is c onsidered.